DNA

Part:BBa_K4373002:Design

Designed by: Mandy Hobusch   Group: iGEM22_Lund   (2022-09-24)


Glyphosate dehydrogenase goxA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal EcoRI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal EcoRI site found at 943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
    Illegal EcoRI site found at 943
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal EcoRI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal EcoRI site found at 943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 300


Design Notes

The part was codon optimizied for Bacillus subtilis.


Source

The gene sequence was obtained from NCBI gene bank [3].


References

[1] Lopes Catão, A. J., & López-Castillo, A. (2018). On the degradation pathway of glyphosate and glycine. Environmental Science: Processes & Impacts, 20(8), 1148–1157. https://doi.org/10.1039/c8em00119g </p>

[2] Quinn, J. P., & Dick, J. E. (1994, October 24). Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradatio. Applied Microbiology Biotechnology, 43, 545–550.

[3] U.S. National Library of Medicine. (n.d.). Uncultured bacterium clone pgoxa fad-dependent glyphosate oxidase (GOX - nucleotide - NCBI. National Center for Biotechnology Information. Retrieved September 24, 2022, from https://www.ncbi.nlm.nih.gov/nucleotide/GU479462.1?report=genbank&log%24=nucltop&blast_rank=6&RID=JXJK73J3013