![](https://parts.igem.org/images/partbypart/icon_dna.png)
Part:BBa_K4373002:Design
Glyphosate dehydrogenase goxA
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 300
Design Notes
The part was codon optimizied for Bacillus subtilis.
Source
The gene sequence was obtained from NCBI gene bank [3].
References
[1] Lopes Catão, A. J., & López-Castillo, A. (2018). On the degradation pathway of glyphosate and glycine. Environmental Science: Processes & Impacts, 20(8), 1148–1157. https://doi.org/10.1039/c8em00119g </p>
[2] Quinn, J. P., & Dick, J. E. (1994, October 24). Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradatio. Applied Microbiology Biotechnology, 43, 545–550.
[3] U.S. National Library of Medicine. (n.d.). Uncultured bacterium clone pgoxa fad-dependent glyphosate oxidase (GOX - nucleotide - NCBI. National Center for Biotechnology Information. Retrieved September 24, 2022, from https://www.ncbi.nlm.nih.gov/nucleotide/GU479462.1?report=genbank&log%24=nucltop&blast_rank=6&RID=JXJK73J3013